Composite

Part:BBa_M36700:Design

Designed by: Katie Lund and Wyatt Woodson   Group: Stanford BIOE44 - S11   (2011-05-03)

PaceBAK w/ Test Actuator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1033


Design Notes

This part will test the efficacy of PaceBAK, the aceBAK promoter sequence to which IclR binds, in order to ensure that there isn't an issue with the promoter region itself. Though there will not be equal levels of IclR and the plasmid containing PaceBAK, we expect under high pyruvate levels there should be enough restriction to witness a decline in Gemini expression.

The part will function by expressing Gemini in the absence of IclR and Pyruvate. If there is adequate amounts of pyruvate-stabilized IclR, however, the promoter region will be inhibited and Gemini will not be expressed.

Source

See individual parts

References